ChT antibody (C-Term)

Catalog No. ABIN2781677

The Rabbit Polyclonal anti-ChT antibody (ABIN2781677) specifically detects ChT in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog and Horse samples.

Quick Overview for ChT antibody (C-Term) (ABIN2781677)

Target: ChT (High Affinity Choline Transporter (ChT))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ChT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ChT Antibody

Binding Specificity: C-Term

Purpose: SLC5A7 Antibody - C-terminal region

Sequence: ILVKNENIKL DELALVKPRQ SMTLSSTFTN KEAFLDVDSS PEGSGTEDNL

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SLC5A7. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of human SLC5A7

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44025-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ChT

Target: ChT (High Affinity Choline Transporter (ChT))

Alternative Name: SLC5A7

Background:

Background Information: This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants.

Gene Name: solute carrier family 5 (sodium/choline cotransporter), member 7

Alternative Symbols: CHT, CHT1, CMS20, HMN7A

Protein Name: high affinity choline transporter 1

Molecular Weight: 63 kDa

Gene ID: 60482

NCBI Accession: NP_001291934

UniProt: Q9GZV3