HFE antibody (N-Term)

Catalog No. ABIN2781799

The Rabbit Polyclonal anti-HFE antibody is suitable to detect HFE in samples from Human, Mouse, Rat, Cow, Horse and Rabbit. It has been validated for WB.

Quick Overview for HFE antibody (N-Term) (ABIN2781799)

Target: HFE (Hemochromatosis (HFE))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HFE antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HFE Antibody

Binding Specificity: N-Term

Purpose: HFE Antibody - N-terminal region

Sequence: MGASEQDLGL SLFEALGYVD DQLFVFYDHE SRRVEPRTPW VSSRISSQMW

Predicted Reactivity: Cow: 79%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against HFE. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HFE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44283-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HFE

Target: HFE (Hemochromatosis (HFE))

Alternative Name: HFE

Background:

Background Information: HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in its gene.

Gene Name: Hemochromatosis

Alternative Symbols: HH, HFE1, HLA-H, MVCD7, TFQTL2

Protein Name: Hereditary hemochromatosis protein

Molecular Weight: 40

Gene ID: 3077

NCBI Accession: NP_000401

UniProt: Q30201

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process