HFE antibody (C-Term)

Catalog No. ABIN2781800

The Rabbit Polyclonal anti-HFE antibody is suitable to detect HFE in samples from Human, Rat, Cow, Horse, Dog, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for HFE antibody (C-Term) (ABIN2781800)

Target: HFE (Hemochromatosis (HFE))

Reactivity: Human, Rat, Cow, Horse, Dog, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HFE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HFE Antibody

Binding Specificity: C-Term

Purpose: HFE Antibody - C-terminal region

Sequence: FEPKDVLPNG DGTYQGWITL AVPPGEEQRY TCQVEHPGLD QPLIVIWEPS

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 86%, Human: 100%, Rat: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against HFE. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HFE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8102334

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HFE

Target: HFE (Hemochromatosis (HFE))

Alternative Name: HFE

Background:

Background Information: HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in its gene.The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least eleven alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.

Gene Name: Hemochromatosis

Alternative Symbols: HH, HFE1, HLA-H, MVCD7, TFQTL2

Protein Name: Hereditary hemochromatosis protein

Molecular Weight: 28kDa

Gene ID: 3077

NCBI Accession: NP_620577

UniProt: Q30201

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process