UGT1A1 antibody (N-Term)

Catalog No. ABIN2781804

The Rabbit Polyclonal anti-UGT1A1 antibody (ABIN2781804) specifically detects UGT1A1 in WB. The antibody is reactive with Human, Mouse, Rat and Horse samples.

Quick Overview for UGT1A1 antibody (N-Term) (ABIN2781804)

Target: UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1))

Reactivity: Human, Mouse, Rat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UGT1A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UGT1A1 Antibody

Binding Specificity: N-Term

Purpose: UGT1A1 Antibody - N-terminal region

Sequence: DGSHWLSMLG AIQQLQQRGH EIVVLAPDAS LYIRDGAFYT LKTYPVPFQR

Predicted Reactivity: Horse: 86%, Human: 100%, Mouse: 79%, Rat: 82%

Characteristics: This is a rabbit polyclonal antibody against UGT1A1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UGT1A1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44292-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for UGT1A1

Target: UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1))

Alternative Name: UGT1A1

Background:

Background Information: UGT1A1 is a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: UDP glucuronosyltransferase 1 family, polypeptide A1

Alternative Symbols: GNT1, UGT1, UDPGT, UGT1A, HUG-BR1, BILIQTL1, UDPGT 1-1

Protein Name: UDP-glucuronosyltransferase 1-1

Molecular Weight: 57kDa

Gene ID: 54658

NCBI Accession: NP_000454

UniProt: P22309

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha