B4GALT2 antibody (Middle Region)

Catalog No. ABIN2781897

The Rabbit Polyclonal anti-B4GALT2 antibody (ABIN2781897) specifically detects B4GALT2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Goat and Sheep samples.

Quick Overview for B4GALT2 antibody (Middle Region) (ABIN2781897)

Target: B4GALT2 (UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 2 (B4GALT2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Goat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B4GALT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-B4GALT2 Antibody

Binding Specificity: Middle Region

Sequence: RLLIEFTSPM PLERVQRENP GVLMGGRYTP PDCTPAQTVA VIIPFRHREH

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 77%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 77%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against B4GALT2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human B4GALT2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 372 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for B4GALT2

Target: B4GALT2 (UDP-Gal:betaGlcNAc beta 1,4- Galactosyltransferase, Polypeptide 2 (B4GALT2))

Alternative Name: B4GALT2

Background: This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose, all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Two transcript variants encoding the same protein have been found for this gene.
Alias Symbols: B4Gal-T2, B4Gal-T3, beta4Gal-T2
Protein Interaction Partner: PFDN6,
Protein Size: 372

Molecular Weight: 42 kDa

Gene ID: 8704

NCBI Accession: NM_001005417, NP_001005417

UniProt: O60909

Pathways: Glycosaminoglycan Metabolic Process