PODXL antibody (N-Term)

Catalog No. ABIN2781991

The Rabbit Polyclonal anti-PODXL antibody is suitable to detect PODXL in samples from Human. It has been validated for WB.

Quick Overview for PODXL antibody (N-Term) (ABIN2781991)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PODXL Antibody

Binding Specificity: N-Term

Purpose: PODXL Antibody - N-terminal region

Sequence: TTTVATSTAT AKPNTTSSQN GAEDTTNSGG KSSHSVTTDL TSTKAEHLTT

Predicted Reactivity: Human: 100%

Characteristics: This is a rabbit polyclonal antibody against PODXL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PODXL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108407

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: PODXL

Background:

Background Information: PODXL is a member of the sialomucin protein family. PODXL was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of PODXL include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.

Gene Name: Podocalyxin-like

Alternative Symbols: PC, PDX, PCLP, Gp200, gp135, PCLP-1, PODXL1

Protein Name: Podocalyxin

Molecular Weight: 53kDa

Gene ID: 5420

NCBI Accession: NP_005388

UniProt: Q53ER6

Pathways: Tube Formation