AREL1 antibody (N-Term)

Catalog No. ABIN2782048

There is 1 publication for this product available. The Rabbit Polyclonal anti-AREL1 antibody is suitable to detect AREL1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). It has been validated for WB and IHC.

Quick Overview for AREL1 antibody (N-Term) (ABIN2782048)

Target: AREL1 (Apoptosis Resistant E3 Ubiquitin Protein Ligase 1 (AREL1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AREL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-AREL1 Antibody

Binding Specificity: N-Term

Purpose: KIAA0317 Antibody - N-terminal region

Sequence: LTCGQPHTLQ IVPRDEYDNP TNNSMSLRDE HNYTLSIHEL GPQEEESTGV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against KIAA0317. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KIAA0317

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44874-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-AREL1 antibody (ABIN2782048)

Lear, McKelvey, Rajbhandari, Dunn, Coon, Connelly, Zhao, Kass, Zhang, Liu, Chen: "Ubiquitin E3 ligase FIEL1 regulates fibrotic lung injury through SUMO-E3 ligase PIAS4." in: The Journal of experimental medicine, Vol. 213, Issue 6, pp. 1029-46, (2016) (PubMed).

Target Details for AREL1

Target: AREL1 (Apoptosis Resistant E3 Ubiquitin Protein Ligase 1 (AREL1))

Alternative Name: KIAA0317

Background:

Background Information: KIAA0317 contains 1 filamin repeat and 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. The exact function of KIAA0317 remains unknown.

Gene Name: KIAA0317

Alternative Symbols: FIEL1, KIAA0317

Protein Name: Protein KIAA0317

Molecular Weight: 94kDa

Gene ID: 9870

NCBI Accession: NP_001034568

UniProt: O15033