Indian Hedgehog antibody (N-Term)

Catalog No. ABIN2782160

Cited in 1 publication. The Rabbit Polyclonal anti-Indian Hedgehog antibody (ABIN2782160) specifically detects Indian Hedgehog in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Guinea Pig, Cow, Goat, Horse, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for Indian Hedgehog antibody (N-Term) (ABIN2782160)

Target: Indian Hedgehog (IHH)

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Goat, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Indian Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Indian Hedgehog Antibody

Binding Specificity: N-Term

Purpose: IHH Antibody - N-terminal region

Sequence: AAWGCGPGRV VGSRRRPPRK LVPLAYKQFS PNVPEKTLGA SGRYEGKIAR

Predicted Reactivity: Cow: 86%, Dog: 86%, Goat: 86%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against IHH. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human IHH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8103013

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Indian Hedgehog antibody (ABIN2782160)

Mirza, Qiao, Tateyama, Miyamoto, Xiuli, Seo: "3?-Hydroxysterol-Delta24 reductase plays an important role in long bone growth by protecting chondrocytes from reactive oxygen species." in: Journal of bone and mineral metabolism, Vol. 30, Issue 2, pp. 144-53, (2012) (PubMed).

Target Details for Indian Hedgehog

Target: Indian Hedgehog (IHH)

Alternative Name: IHH

Background:

Background Information: IHH is an intercellular signal essential for a variety of patterning events during development. It binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. It is implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones and induces the expression of parathyroid hormone-related protein (PTHRP).

Gene Name: Indian hedgehog

Alternative Symbols: BDA1, HHG2

Protein Name: Indian hedgehog protein

Molecular Weight: 45 kDa

Gene ID: 3549

NCBI Accession: NP_002172

UniProt: Q14623

Pathways: Hedgehog Signaling