ZP2 antibody (C-Term)

Catalog No. ABIN2782230

The Rabbit Polyclonal anti-ZP2 antibody (ABIN2782230) specifically detects ZP2 in WB. The antibody is reactive with Human, Rat, Horse, Guinea Pig, Rabbit, Cow and Dog samples.

Quick Overview for ZP2 antibody (C-Term) (ABIN2782230)

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Reactivity: Human, Rat, Horse, Guinea Pig, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZP2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ZP2 Antibody

Binding Specificity: C-Term

Purpose: ZP2 Antibody - C-terminal region

Sequence: PDSFPQWNVV VDGCAYDLDN YQTTFHPVGS SVTHPDHYQR FDMKAFAFVS

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ZP2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ZP2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8117258

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ZP2

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Alternative Name: ZP2

Background:

Background Information: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. ZP2 is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.

Gene Name: Zona pellucida glycoprotein 2 (sperm receptor)

Alternative Symbols: ZPA, Zp-2, OOMD6

Protein Name: Zona pellucida sperm-binding protein 2

Molecular Weight: 68kDa

Gene ID: 7783

NCBI Accession: NP_003451

UniProt: Q05996