Dysferlin antibody (N-Term)

Catalog No. ABIN2782234

The Rabbit Polyclonal anti-Dysferlin antibody is suitable to detect Dysferlin in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for Dysferlin antibody (N-Term) (ABIN2782234)

Target: Dysferlin (DYSF)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dysferlin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Dysferlin Antibody

Binding Specificity: N-Term

Purpose: DYSF Antibody - N-terminal region

Sequence: DKPQDFQIRV QVIEGRQLPG VNIKPVVKVT AAGQTKRTRI HKGNSPLFNE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against DYSF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DYSF

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP45477-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Dysferlin

Target: Dysferlin (DYSF)

Alternative Name: DYSF

Background:

Background Information: DYSF belongs to the ferlin family and is a skeletal muscle protein found associated with the sarcolemma. It is involved in muscle contraction and contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be in

Gene Name: Dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)

Alternative Symbols: MMD1, FER1L1, LGMD2B, LGMDR2

Protein Name: Dysferlin

Molecular Weight: 237kDa

Gene ID: 8291

NCBI Accession: NP_003485

UniProt: O75923