GALNT4 antibody (C-Term)

Catalog No. ABIN2782249

Product Details anti-GALNT4 Antibody

Target: GALNT4 (UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 4 (GalNAc-T4) (GALNT4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALNT4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ANLSLFGCHG QGGNQFFEYT SNKEIRFNSV TELCAEVPEQ KNYVGMQNCP

Predicted Reactivity: Cow: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 93%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against GALNT4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GALNT4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 578 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GALNT4

Target: GALNT4 (UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 4 (GalNAc-T4) (GALNT4))

Alternative Name: GALNT4 (GALNT4 Products)

Synonyms: GALNAC-T4 antibody, GALNACT4 antibody, AV011803 antibody, polypeptide N-acetylgalactosaminyltransferase 4 antibody, polypeptide N-acetylgalactosaminyltransferase 4 L homeolog antibody, GALNT4 antibody, galnt4.L antibody, Galnt4 antibody

Background: GALNT4 catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 Molecule. The coding region of this gene is contained within a single exon.
Alias Symbols: GALNAC-T4, GalNAcT4, GALNACT4
Protein Interaction Partner: ELAVL1, SELPLG, MUC1,
Protein Size: 578

Molecular Weight: 67 kDa

Gene ID: 8693

NCBI Accession: NM_003774, NP_003765

UniProt: Q8N4A0