CBS antibody (N-Term)

Catalog No. ABIN2782348

This Rabbit Polyclonal antibody specifically detects CBS in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae. It has been mentioned in 8+ publications

Quick Overview for CBS antibody (N-Term) (ABIN2782348)

Target: CBS (Cystathionine-beta-Synthase (CBS))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CBS Antibody

Binding Specificity: N-Term

Purpose: CBS Antibody - N-terminal region

Sequence: RCIIVMPEKM SSEKVDVLRA LGAEIVRTPT NARFDSPESH VGVAWRLKNE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 86%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CBS. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CBS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098222

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CBS antibody (ABIN2782348)

Yamane, Kanno, Nakamura, Fujino, Murayama: "Hydrogen sulfide-mediated regulation of contractility in the mouse ileum with electrical stimulation: roles of L-cysteine, cystathionine β-synthase, and K+ channels." in: European journal of pharmacology, Vol. 740, pp. 112-20, (2014) (PubMed).

Geillinger, Rathmann, Köhrle, Fiamoncini, Daniel, Kipp: "Hepatic metabolite profiles in mice with a suboptimal selenium status." in: The Journal of nutritional biochemistry, Vol. 25, Issue 9, pp. 914-22, (2014) (PubMed).

Porteus, Abdallah, Pollack, Kumai, Kwong, Yew, Milsom, Perry: "The role of hydrogen sulphide in the control of breathing in hypoxic zebrafish (Danio rerio)." in: The Journal of physiology, Vol. 592, Issue 14, pp. 3075-88, (2014) (PubMed).

Dahlhoff, Worsch, Sailer, Hummel, Fiamoncini, Uebel, Obeid, Scherling, Geisel, Bader, Daniel: "Methyl-donor supplementation in obese mice prevents the progression of NAFLD, activates AMPK and decreases acyl-carnitine levels." in: Molecular metabolism, Vol. 3, Issue 5, pp. 565-80, (2014) (PubMed).

Fernandes, Ribeiro, Martínez, Orensanz, Barahona, Martínez-Sáenz, Recio, Benedito, Bustamante, Carballido, García-Sacristán, Prieto, Hernández: "Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck." in: The Journal of urology, Vol. 189, Issue 4, pp. 1567-73, (2013) (PubMed).

Markand, Tawfik, Ha, Gnana-Prakasam, Sonne, Ganapathy, Sen, Xian, Smith: "Cystathionine beta synthase expression in mouse retina." in: Current eye research, Vol. 38, Issue 5, pp. 597-604, (2013) (PubMed).

Dahlhoff, Desmarchelier, Sailer, Fürst, Haag, Ulbrich, Hummel, Obeid, Geisel, Bader, Daniel: "Hepatic methionine homeostasis is conserved in C57BL/6N mice on high-fat diet despite major changes in hepatic one-carbon metabolism." in: PLoS ONE, Vol. 8, Issue 3, pp. e57387, (2013) (PubMed).

Spellman, Ahmed, Dubach, Gardiner: "Expression of trisomic proteins in Down syndrome model systems." in: Gene, Vol. 512, Issue 2, pp. 219-25, (2012) (PubMed).

Target Details for CBS

Target: CBS (Cystathionine-beta-Synthase (CBS))

Alternative Name: CBS

Background:

Background Information: CBS is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.The protein encoded by this gene is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.

Gene Name: Cystathionine-beta-synthase

Alternative Symbols: CBSL, HIP4

Protein Name: Cystathionine beta-synthase

Molecular Weight: 61 kDa

Gene ID: 875

NCBI Accession: NP_000062

UniProt: P35520