Deoxyuridine Triphosphatase (DUT) (C-Term) antibody

Catalog No. ABIN2782436

This Rabbit Polyclonal antibody specifically detects in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Saccharomyces cerevisiae, Zebrafish (Danio rerio) and Rabbitand has been mentioned in 1 publication.

Quick Overview for Deoxyuridine Triphosphatase (DUT) (C-Term) antibody (ABIN2782436)

Target: Deoxyuridine Triphosphatase (DUT)

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Saccharomyces cerevisiae, Zebrafish (Danio rerio), Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: C-Term

Sequence: NFGKEKFEVK KGDRIAQLIC ERIFYPEIEE VQALDDTERG SGGFGSTGKN

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Yeast: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against DUT. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DUT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 252 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Studebaker, Lafuse, Kloesel, Williams: "Modulation of human dUTPase using small interfering RNA." in: Biochemical and biophysical research communications, Vol. 327, Issue 1, pp. 306-10, (2005) (PubMed).

Target Details

Target: Deoxyuridine Triphosphatase (DUT)

Alternative Name: DUT

Background: DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Alias Symbols: FLJ20622, dUTPase
Protein Interaction Partner: GDI2, NUDT18, PLEKHF2, C19orf25, MRPL14, LEMD3, UBL4A, RPL38, UBC, CUL3, ESRRG, ESRRA, ESR1, SPATA2, DUT, PPARD, PPARA, CDK1,
Protein Size: 252

Molecular Weight: 19 kDa

Gene ID: 1854

NCBI Accession: NM_001025248, NP_001020419

UniProt: P33316