RRM2 antibody (N-Term)

Catalog No. ABIN2782440

Cited in 1 publication. The Rabbit Polyclonal anti-RRM2 antibody (ABIN2782440) specifically detects RRM2 in WB. The antibody is reactive with Human, Rat, Guinea Pig, Horse, Cow and Dog samples.

Quick Overview for RRM2 antibody (N-Term) (ABIN2782440)

Target: RRM2 (Ribonucleotide Reductase M2 (RRM2))

Reactivity: Human, Rat, Guinea Pig, Horse, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RRM2 Antibody

Binding Specificity: N-Term

Purpose: RRM2 Antibody - N-terminal region

Sequence: PALSGTRVLA SKTARRIFQE PTEPKTKAAA PGVEDEPLLR ENPRRFVIFP

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RRM2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RRM2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46031-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-RRM2 antibody (ABIN2782440)

Lane, Yu, Lackey, Chen, Marzluff, Cook: "Cell cycle-regulated protein abundance changes in synchronously proliferating HeLa cells include regulation of pre-mRNA splicing proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58456, (2013) (PubMed).

Target Details for RRM2

Target: RRM2 (Ribonucleotide Reductase M2 (RRM2))

Alternative Name: RRM2

Background:

Targeted Subunit: M2

Background Information: RRM2 provides the precursors necessary for DNA synthesis. RRM2 catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. RRM2 inhibits Wnt signaling.Ribonucleotide reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. It is composed of 2 non-identical subunits, proteins M1 and M2. Synthesis of M2 is regulated in a cell-cycle dependent fashion. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Ribonucleotide reductase M2

Alternative Symbols: R2, RR2, RR2M, C2orf48

Protein Name: Ribonucleoside-diphosphate reductase subunit M2

Molecular Weight: 45kDa

Gene ID: 6241

NCBI Accession: NP_001025

UniProt: P31350

Pathways: Mitotic G1-G1/S Phases