MTHFD2 antibody (N-Term)

Catalog No. ABIN2782446

This Rabbit Polyclonal antibody specifically detects MTHFD2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Horse, Cow, Zebrafish (Danio rerio) and Saccharomyces cerevisiaeand has been mentioned in 1 publication.

Quick Overview for MTHFD2 antibody (N-Term) (ABIN2782446)

Target: MTHFD2 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase (MTHFD2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Horse, Cow, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTHFD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MTHFD2 Antibody

Binding Specificity: N-Term

Sequence: LPLPEHIDER RICNAVSPDK DVDGFHVINV GRMCLDQYSM LPATPWGVWE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against MTHFD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MTHFD2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 350 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MTHFD2 antibody (ABIN2782446)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for MTHFD2

Target: MTHFD2 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase (MTHFD2))

Alternative Name: MTHFD2

Background: MTHFD2 is a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD.This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.
Alias Symbols: NMDMC
Protein Interaction Partner: NAGK, HUWE1, UBC, MDM2, nef, DHX40, GCC1, DHX29, CUL3, ELAVL1, MINOS1, PARP3, PSMD13,
Protein Size: 350

Molecular Weight: 35 kDa

Gene ID: 10797

NCBI Accession: NM_006636, NP_006627

UniProt: P13995