DDT antibody (N-Term)

Catalog No. ABIN2782455

This Rabbit Polyclonal antibody specifically detects DDT in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow, Guinea Pig, Horse and Rabbit.It has been mentioned in 1 publication

Quick Overview for DDT antibody (N-Term) (ABIN2782455)

Target: DDT (D-Dopachrome Tautomerase (DDT))

Reactivity: Human, Mouse, Rat, Pig, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDT Antibody

Binding Specificity: N-Term

Purpose: DDT Antibody - N-terminal region

Sequence: PFLELDTNLP ANRVPAGLEK RLCAAAASIL GKPADRVNVT VRPGLAMALS

Predicted Reactivity: Cow: 85%, Guinea Pig: 75%, Horse: 93%, Human: 100%, Mouse: 77%, Pig: 77%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against DDT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46052-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDT antibody (ABIN2782455)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for DDT

Target: DDT (D-Dopachrome Tautomerase (DDT))

Alternative Name: DDT

Background:

Background Information: D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. It is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22.D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22.

Gene Name: D-dopachrome tautomerase

Alternative Symbols: D-DT, DDCT, MIF2, MIF-2

Protein Name: D-dopachrome decarboxylase Ensembl ENSP00000384866

Molecular Weight: 13kDa

Gene ID: 1652

NCBI Accession: NP_001346

UniProt: B5MCS6