CTH antibody (N-Term)

Catalog No. ABIN2782463

Cited in 3+ publications. The Rabbit Polyclonal anti-CTH antibody (ABIN2782463) specifically detects CTH in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse and Pig samples.

Quick Overview for CTH antibody (N-Term) (ABIN2782463)

Target: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CTH Antibody

Binding Specificity: N-Term

Purpose: CTH Antibody - N-terminal region

Sequence: VPPISLSTTF KQGAPGQHSG FEYSRSGNPT RNCLEKAVAA LDGAKYCLAF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CTH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CTH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46067-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CTH antibody (ABIN2782463)

Perry, Kumai, Porteus, Tzaneva, Kwong: "An emerging role for gasotransmitters in the control of breathing and ionic regulation in fish." in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, Vol. 186, Issue 2, pp. 145-59, (2016) (PubMed).

Kumai, Porteus, Kwong, Perry: "Hydrogen sulfide inhibits Na+ uptake in larval zebrafish, Danio rerio." in: Pflügers Archiv : European journal of physiology, Vol. 467, Issue 4, pp. 651-64, (2015) (PubMed).

Porteus, Abdallah, Pollack, Kumai, Kwong, Yew, Milsom, Perry: "The role of hydrogen sulphide in the control of breathing in hypoxic zebrafish (Danio rerio)." in: The Journal of physiology, Vol. 592, Issue 14, pp. 3075-88, (2014) (PubMed).

Target Details for CTH

Target: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Alternative Name: CTH

Background:

Background Information: CTH is a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in its gene cause cystathioninuria.This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Gene Name: Cystathionase (cystathionine gamma-lyase)

Alternative Symbols: MGC9471

Protein Name: Cystathionine gamma-lyase

Molecular Weight: 45 kDa

Gene ID: 1491

NCBI Accession: NP_001893

UniProt: P32929

Pathways: ER-Nucleus Signaling, Warburg Effect