HSPA4 antibody (Middle Region)

Catalog No. ABIN502697

The Rabbit Polyclonal anti-HSPA4 antibody is suitable to detect HSPA4 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio) and Rabbit. It has been validated for WB.

Quick Overview for HSPA4 antibody (Middle Region) (ABIN502697)

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio), Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPA4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSPA4 Antibody

Binding Specificity: Middle Region

Purpose: HSPA4 Antibody - middle region

Sequence: PIKIRFQESE ERPKLFEELG KQIQQYMKII SSFKNKEDQY DHLDAADMTK

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against HSPA4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HSPA4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46074-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HSPA4

Target: HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))

Alternative Name: HSPA4

Background:

Background Information: HSPA4(Hsp70) belongs to the heat shock protein 70 family. It was isolated as a putative Rictor interacting protein and interaction with membranes acts as a platform for its release into the extracellular environment during its recovery from stress. Hsp70 gene expression in Rheumatoid Arthitis-affected synovial tissue is followed by Hsp70 cell surface expression on fibroblast-like synovial cells growing from RA synovial tissue. Serum Hsp70 levels were increased in Systemic sclerosis patients, and associated with pulmonary fibrosis, skin sclerosis, renal vascular damage, oxidative stress, and inflammation.

Gene Name: Heat shock 70 kDa protein 4

Alternative Symbols: RY, APG-2, HSPH2, hsp70, hsp70RY, HEL-S-5a, HS24/P52

Protein Name: Heat shock 70 kDa protein 4

Molecular Weight: 94kDa

Gene ID: 3308

NCBI Accession: NP_002145

UniProt: P34932