PPIF antibody (Middle Region)

Catalog No. ABIN2782510

The Rabbit Polyclonal anti-PPIF antibody (ABIN2782510) specifically detects PPIF in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse, Saccharomyces cerevisiae, Sheep and Zebrafish (Danio rerio) samples.

Quick Overview for PPIF antibody (Middle Region) (ABIN2782510)

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse, Saccharomyces cerevisiae, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPIF Antibody

Binding Specificity: Middle Region

Purpose: PPIF Antibody - middle region

Sequence: GSTFHRVIPS FMCQAGDFTN HNGTGGKSIY GSRFPDENFT LKHVGPGVLS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 92%, Yeast: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against PPIF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PPIF

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8107827

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PPIF

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Alternative Name: PPIF

Background:

Background Information: PPIF is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

Gene Name: Peptidylprolyl isomerase F

Alternative Symbols: CYP3, CypD, CyP-M, Cyp-D

Protein Name: Peptidyl-prolyl cis-trans isomerase F, mitochondrial

Molecular Weight: 19kDa

Gene ID: 10105

NCBI Accession: NP_005720

UniProt: P30405

Pathways: Proton Transport, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity