MTHFD1 antibody (Middle Region)

Catalog No. ABIN2782515

The Rabbit Polyclonal anti-MTHFD1 antibody (ABIN2782515) specifically detects MTHFD1 in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Rabbit, Horse, Zebrafish (Danio rerio), Pig, Dog, Guinea Pig, Cow and Saccharomyces cerevisiae samples.

Quick Overview for MTHFD1 antibody (Middle Region) (ABIN2782515)

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Reactivity: Human, Mouse, Rat, Rabbit, Horse, Zebrafish (Danio rerio), Pig, Dog, Guinea Pig, Cow, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTHFD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MTHFD1 Antibody

Binding Specificity: Middle Region

Sequence: CMAKTHLSLS HNPEQKGVPT GFILPIRDIR ASVGAGFLYP LVGTMSTMPG

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against MTHFD1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MTHFD1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 935 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MTHFD1

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Alternative Name: MTHFD1

Background: MTHFD1 possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MTHFC, MTHFD
Protein Interaction Partner: UBC, FUS, SUMO2, SUMO3, NEDD8, MDM2, RPA3, RPA2, RPA1, RNF2, RPS21, MAT2A, UBQLN1, FBXO6, UBD, ADRB2, CD81, VCAM1, HSP90AA1, HSP90AB1, PRKCDBP, SFXN1, TMEM165, IARS2, SARS2, SF3B3, FAF2, PPT2, SNRNP70, RPL32, MRPL23, ALDH18A1, PRPH, PC, ICT1, GAA, ACSL3,
Protein Size: 935

Molecular Weight: 101 kDa

Gene ID: 4522

NCBI Accession: NM_005956, NP_005947

UniProt: P11586

Pathways: Methionine Biosynthetic Process