VARS antibody (Middle Region)

Catalog No. ABIN2782516

This anti-VARS antibody is a Rabbit Polyclonal antibody detecting VARS in WB. Suitable for Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig and Pig. This Primary Antibody has been cited in 1 publication.

Quick Overview for VARS antibody (Middle Region) (ABIN2782516)

Target: VARS (Valyl-tRNA Synthetase (VARS))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VARS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VARS Antibody

Binding Specificity: Middle Region

Sequence: AQRLRERRAA SGYPVKVPLE VQEADEAKLQ QTEAELRKVD EAIALFQKML

Predicted Reactivity: Cow: 100%, Dog: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against VARS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human VARS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1264 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-VARS antibody (ABIN2782516)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for VARS

Target: VARS (Valyl-tRNA Synthetase (VARS))

Alternative Name: VARS

Background: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. VARS belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: G7A, VARS2, VARS1
Protein Interaction Partner: CALCOCO2, SUMO2, SART3, STAU1, UBC, EED, CCT4, KNTC1, AIMP1, CCT3, QARS, EEF1G, EEF1D, EEF1B2, FBXO6, YWHAQ, MAPK11, VCAM1, ITGA4, FN1, LARS, CAD, UBD, SHC1, CUL3, CDK2, CHD7, CACNA1A, ATG101, OTUD5, DMWD, EEF1A1, EEF1A2, TRAF6, TFE3, HLA-B, EPB41,
Protein Size: 1264

Molecular Weight: 140 kDa

Gene ID: 7407

NCBI Accession: NM_006295, NP_006286

UniProt: P26640