VARS antibody (Middle Region)

Catalog No. ABIN2782517

Cited in 2+ publications. The Rabbit Polyclonal anti-VARS antibody (ABIN2782517) specifically detects VARS in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig, Zebrafish (Danio rerio) and Saccharomyces cerevisiae samples.

Quick Overview for VARS antibody (Middle Region) (ABIN2782517)

Target: VARS (Valyl-tRNA Synthetase (VARS))

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VARS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VARS Antibody

Binding Specificity: Middle Region

Purpose: VARS Antibody - middle region

Sequence: VFDEFVDMDF GTGAVKITPA HDQNDYEVGQ RHGLEAISIM DSRGALINVP

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 86%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against VARS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human VARS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46151-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-VARS antibody (ABIN2782517)

Duarte, Videira: "Defective valyl-tRNA synthetase hampers the mitochondrial respiratory chain in Neurospora crassa." in: The Biochemical journal, Vol. 448, Issue 3, pp. 297-306, (2012) (PubMed).

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for VARS

Target: VARS (Valyl-tRNA Synthetase (VARS))

Alternative Name: VARS

Background:

Background Information: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. VARS belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Valyl-tRNA synthetase

Alternative Symbols: G7A, VARS, VARS2, NDMSCA

Protein Name: Valine--tRNA ligase

Molecular Weight: 140kDa

Gene ID: 7407

NCBI Accession: NP_006286

UniProt: P26640