PFAS antibody (N-Term)

Catalog No. ABIN2782529

This Rabbit Polyclonal antibody specifically detects PFAS in WB. It exhibits reactivity toward Human, Mouse, Cow, Guinea Pig, Horse and Rabbit. It has been mentioned in 2+ publications

Quick Overview for PFAS antibody (N-Term) (ABIN2782529)

Target: PFAS (Phosphoribosylformylglycinamidine Synthase (PFAS))

Reactivity: Human, Mouse, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFAS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PFAS Antibody

Binding Specificity: N-Term

Purpose: PFAS Antibody - N-terminal region

Sequence: ESIMSTQESS NPNNVLKFCD NSSAIQGKEV RFLRPEDPTR PSRFQQQQGL

Predicted Reactivity: Cow: 83%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 79%

Characteristics: This is a rabbit polyclonal antibody against PFAS. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PFAS

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46181-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PFAS antibody (ABIN2782529)

French, Zhao, An, Niessen, Deng, Cravatt, Benkovic: "Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 7, pp. 2528-33, (2013) (PubMed).

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for PFAS

Target: PFAS (Phosphoribosylformylglycinamidine Synthase (PFAS))

Alternative Name: PFAS

Background:

Background Information: Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis. Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis.

Gene Name: Phosphoribosylformylglycinamidine synthase

Alternative Symbols: PURL, FGAMS, GATD8, FGARAT, FGAR-AT

Protein Name: Phosphoribosylformylglycinamidine synthase

Molecular Weight: 145kDa

Gene ID: 5198

NCBI Accession: NP_036525

UniProt: O15067