NIT2 antibody (Middle Region)

Catalog No. ABIN2782571

The Rabbit Polyclonal anti-NIT2 antibody is suitable to detect NIT2 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Saccharomyces cerevisiae and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for NIT2 antibody (Middle Region) (ABIN2782571)

Target: NIT2 (Nitrilase Family, Member 2 (NIT2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NIT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NIT2 Antibody

Binding Specificity: Middle Region

Purpose: NIT2 Antibody - middle region

Sequence: VAKECSIYLI GGSIPEEDAG KLYNTCAVFG PDGTLLAKYR KIHLFDIDVP

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 79%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NIT2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NIT2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46292-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NIT2

Target: NIT2 (Nitrilase Family, Member 2 (NIT2))

Alternative Name: NIT2

Background:

Background Information: NIT2 belongs to the UPF0012 family and contains 1 CN hydrolase domain. Overexpression of NIT2 decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

Gene Name: Nitrilase family, member 2

Alternative Symbols: HEL-S-8a

Protein Name: Omega-amidase NIT2

Molecular Weight: 30kDa

Gene ID: 56954

NCBI Accession: NP_064587

UniProt: Q9NQR4