NMT1 antibody (N-Term)

Catalog No. ABIN2782574

This anti-NMT1 antibody is a Rabbit Polyclonal antibody detecting NMT1 in WB. Suitable for Human, Mouse, Rat, Cow, Guinea Pig, Pig, Rabbit, Zebrafish (Danio rerio), Dog and Horse. This Primary Antibody has been cited in 1 publication.

Quick Overview for NMT1 antibody (N-Term) (ABIN2782574)

Target: NMT1 (N-Myristoyltransferase 1 (NMT1))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Pig, Rabbit, Zebrafish (Danio rerio), Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NMT1 Antibody

Binding Specificity: N-Term

Sequence: TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against NMT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NMT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 496 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NMT1 antibody (ABIN2782574)

Takamune, Gota, Misumi, Tanaka, Okinaka, Shoji: "HIV-1 production is specifically associated with human NMT1 long form in human NMT isozymes." in: Microbes and infection / Institut Pasteur, Vol. 10, Issue 2, pp. 143-50, (2008) (PubMed).

Target Details for NMT1

Target: NMT1 (N-Myristoyltransferase 1 (NMT1))

Alternative Name: NMT1

Background: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1).
Alias Symbols: NMT
Protein Interaction Partner: C14orf166, RTCB, DIMT1, NELFB, EDC4, IGF2BP3, PDCD6, POLR1C, EIF2B2, EIF2B3, YBX3, RPL38, RPL23A, RPL19, RFC4, QARS, PA2G4, YBX1, HNRNPM, MRE11A, MAP2, ILF2, IARS, HNRNPU, FLII, FKBP3, DYNC1LI2, DHX9, DDX1, ABCF1, nef, UBC, CSNK2A1, gag, ARL5A, CABP2, MAR
Protein Size: 496

Molecular Weight: 57 kDa

Gene ID: 4836

NCBI Accession: NM_021079, NP_066565

UniProt: P30419

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling