MRPS12 antibody (N-Term)

Catalog No. ABIN2782575

Product Details anti-MRPS12 Antibody

Target: MRPS12 (Mitochondrial Ribosomal Protein S12 (MRPS12))

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS12 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LVPRLWATCS MATLNQMHRL GPPKRPPRKL GPTEGRPQLK GVVLCTFTRK

Predicted Reactivity: Cow: 86%, Dog: 91%, Guinea Pig: 86%, Horse: 79%, Human: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against MRPS12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPS12

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 138 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPS12

Target: MRPS12 (Mitochondrial Ribosomal Protein S12 (MRPS12))

Alternative Name: MRPS12 (MRPS12 Products)

Background: Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPS12 is the 28S subunit protein that belongs to the ribosomal protein S12P family. The protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene, all three variants encode the same protein.
Alias Symbols: MPR-S12, MT-RPS12, RPMS12, RPS12, RPSM12
Protein Interaction Partner: UBC, LRIF1, SPINK7, C14orf1, UNC119, CRMP1,
Protein Size: 138

Molecular Weight: 12 kDa

Gene ID: 6183

NCBI Accession: NM_021107, NP_066930

UniProt: O15235

Pathways: Sensory Perception of Sound