CENPM antibody (Middle Region)

Catalog No. ABIN2782583

The Rabbit Polyclonal anti-CENPM antibody is suitable to detect CENPM in samples from Human, Cow, Dog, Horse, Pig and Rabbit. It has been validated for WB and IF.

Quick Overview for CENPM antibody (Middle Region) (ABIN2782583)

Target: CENPM (Centromere Protein M (CENPM))

Reactivity: Human, Cow, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-CENPM Antibody

Binding Specificity: Middle Region

Purpose: CENPM Antibody - middle region

Sequence: CDLEVEGFRA TMAQRLVRVL QICAGHVPGV SALNLLSLLR SSEGPSLEDL

Predicted Reactivity: Cow: 79%, Dog: 79%, Horse: 93%, Human: 100%, Pig: 93%, Rabbit: 93%

Characteristics: This is a rabbit polyclonal antibody against CENPM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CENPM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46323-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for CENPM

Target: CENPM (Centromere Protein M (CENPM))

Alternative Name: CENPM

Background:

Background Information: The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA replaces histone H3. CENPM is an additional factor required for centromere assembly.The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPM is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM].

Gene Name: Centromere protein M

Alternative Symbols: PANE1, CENP-M, C22orf18

Protein Name: Centromere protein M

Molecular Weight: 20kDa

Gene ID: 79019

NCBI Accession: NP_076958

UniProt: Q9NSP4