CDT1 antibody (C-Term)

Catalog No. ABIN2782592

The Rabbit Polyclonal anti-CDT1 antibody is suitable to detect CDT1 in samples from Human, Mouse, Rat, Pig, Cow, Dog, Rabbit and Saccharomyces cerevisiae. It has been validated for WB and IHC.

Quick Overview for CDT1 antibody (C-Term) (ABIN2782592)

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CDT1 Antibody

Binding Specificity: C-Term

Sequence: PATPPATPPA ASPSALKGVS QDLLERIRAK EAQKQLAQMT RCPEQEQRLQ

Predicted Reactivity: Cow: 93%, Dog: 77%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Yeast: 85%

Characteristics: This is a rabbit polyclonal antibody against CDT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CDT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 546 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CDT1

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Alternative Name: CDT1

Background: CDT1 cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. It binds DNA in a sequence-, strand-, and conformation-independent manner and is a potential oncogene.
Alias Symbols: DUP, RIS2
Protein Interaction Partner: FBXO31, SKP1, RBX1, CUL1, DTL, PCNA, HECW2, CDK2, CDC6, CDC5L, ORC2, ORC1, GMNN, DGCR8, UBC, SKP2, CDK1, CCNB1, CCNA2, FOXO3, DDB1, COPS5, CUL4A, CUL4B, SMARCA5, MCM6, MAPK8, MAPK1, HDAC11, KAT7, CCNA1, DDB2, CDK4, CCNE1, TP63, TP73, EP300, KAT2B, CDT1, U
Protein Size: 546

Molecular Weight: 60 kDa

Gene ID: 81620

NCBI Accession: NM_030928, NP_112190

UniProt: Q9H211

Pathways: MAPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA