Lamin B2 antibody (Middle Region)

Catalog No. ABIN2782595

The Rabbit Polyclonal anti-Lamin B2 antibody is suitable to detect Lamin B2 in samples from Human, Rat, Mouse, Zebrafish (Danio rerio), Horse, Cow, Guinea Pig, Rabbit and Dog. It has been validated for WB.

Quick Overview for Lamin B2 antibody (Middle Region) (ABIN2782595)

Target: Lamin B2 (LMNB2)

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Horse, Cow, Guinea Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Lamin B2 Antibody

Binding Specificity: Middle Region

Purpose: LMNB2 Antibody - middle region

Sequence: EVAMRTVKKS SVMRENENGE EEEEEAEFGE EDLFHQQGDP RTTSRGCYVM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 83%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against LMNB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human LMNB2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46357-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Lamin B2

Target: Lamin B2 (LMNB2)

Alternative Name: LMNB2

Background:

Background Information: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. LMNB2 is one of the two B type proteins, B2. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Lamin B2

Alternative Symbols: EPM9, LMN2, LAMB2, MCPH27

Protein Name: Lamin-B2

Molecular Weight: 68kDa

Gene ID: 84823

NCBI Accession: NP_116126

UniProt: Q03252

Pathways: Apoptosis, Caspase Cascade in Apoptosis