Cadherin 12 antibody (Middle Region)

Catalog No. ABIN2782614

This Rabbit Polyclonal antibody specifically detects Cadherin 12 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Horse and Zebrafish (Danio rerio).

Quick Overview for Cadherin 12 antibody (Middle Region) (ABIN2782614)

Target: Cadherin 12

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Cow, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cadherin 12 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Cadherin 12 Antibody

Binding Specificity: Middle Region

Purpose: CDH12 Antibody - middle region

Sequence: DTQEGVIKLK KPLDFETKKA YTFKVEASNL HLDHRFHSAG PFKDTATVKI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against CDH12. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CDH12

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46400-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cadherin 12

Target: Cadherin 12

Alternative Name: CDH12

Background:

Background Information: CDH12 belongs to the protocadherin protein family, a subfamily of the cadherin superfamily. It consists of an extracellular domain containing 6 cadherin repeats, a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The function of this cellular adhesion protein is undetermined but mouse protocadherin 12 does not bind catenins and appears to have no affect on cell migration or growth.This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.

Gene Name: Cadherin 12, type 2 (N-cadherin 2)

Alternative Symbols: CDHB

Protein Name: Cadherin-12

Molecular Weight: 82kDa

Gene ID: 1010

NCBI Accession: NP_004052

UniProt: P55289