COX15 antibody (N-Term)

Catalog No. ABIN2782632

Cited in 1 publication. The Rabbit Polyclonal anti-COX15 antibody (ABIN2782632) specifically detects COX15 in IHC and WB. The antibody is reactive with Human, Rat, Mouse, Dog, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse and Saccharomyces cerevisiae samples.

Quick Overview for COX15 antibody (N-Term) (ABIN2782632)

Target: COX15 (Cytochrome C Oxidase Assembly Homolog 15 (COX15))

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX15 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-COX15 Antibody

Binding Specificity: N-Term

Purpose: COX15 Antibody - N-terminal region

Sequence: DWHLIKEMKP PTSQEEWEAE FQRYQQFPEF KILNHDMTLT EFKFIWYMEY

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 86%, Rat: 93%, Yeast: 82%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against COX15. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human COX15

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098323

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-COX15 antibody (ABIN2782632)

Swenson, Cannon, Harris, Taylor, Fox, Khalimonchuk: "Analysis of Oligomerization Properties of Heme a Synthase Provides Insights into Its Function in Eukaryotes." in: The Journal of biological chemistry, Vol. 291, Issue 19, pp. 10411-25, (2016) (PubMed).

Target Details for COX15

Target: COX15 (Cytochrome C Oxidase Assembly Homolog 15 (COX15))

Alternative Name: COX15

Background:

Background Information: Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane.Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates several transcript variants diverging in the 3' region including alternate poly A sites. In total, 2 different isoforms are encoded by these variants.

Gene Name: COX15 homolog, cytochrome c oxidase assembly protein (yeast)

Alternative Symbols: MC4DN6, CEMCOX2

Protein Name: Cytochrome c oxidase assembly protein COX15 homolog

Molecular Weight: 46 kDa

Gene ID: 1355

NCBI Accession: NP_004367

UniProt: Q7KZN9