Seladin 1 antibody (N-Term)

Catalog No. ABIN2782831

The Rabbit Polyclonal anti-Seladin 1 antibody is suitable to detect Seladin 1 in samples from Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig and Horse. It has been validated for WB.

Quick Overview for Seladin 1 antibody (N-Term) (ABIN2782831)

Target: Seladin 1 (DHCR24) (24-Dehydrocholesterol Reductase (DHCR24))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Seladin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Seladin 1 Antibody

Binding Specificity: N-Term

Purpose: DHCR24 Antibody - N-terminal region

Sequence: FLLPLSLIFD IYYYVRAWVV FKLSSAPRLH EQRVRDIQKQ VREWKEQGSK

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against DHCR24. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DHCR24

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47072-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Seladin 1

Target: Seladin 1 (DHCR24) (24-Dehydrocholesterol Reductase (DHCR24))

Alternative Name: DHCR24

Background:

Background Information: DHCR24 is a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: 24-dehydrocholesterol reductase

Alternative Symbols: DCE, SELADIN1, Nbla03646, seladin-1

Protein Name: Delta(24)-sterol reductase

Molecular Weight: 58kDa

Gene ID: 1718

NCBI Accession: NP_055577

UniProt: Q15392