CHST15 antibody (Middle Region)

Catalog No. ABIN2782860

Product Details anti-CHST15 Antibody

Target: CHST15 (Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 (CHST15))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST15 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YDNSTDGEPP FLTQDFIHAF QPNARLIVML RDPVERLYSD YLYFASSNKS

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GALNAC4S-6ST. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GALNAC4S-6ST

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 561 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CHST15

Target: CHST15 (Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 (CHST15))

Alternative Name: GALNAC4S-6ST (CHST15 Products)

Background: GALNAC4S-6ST is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. GALNAC4S-6ST may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression, however such results are unclear in vivo.
Alias Symbols: BRAG, DKFZp781H1369, KIAA0598, MGC34346, RP11-47G11.1, GALNAC4S-6ST
Protein Interaction Partner: UBC, RRAGD, LYN, HCK,
Protein Size: 561

Molecular Weight: 65 kDa

Gene ID: 51363

NCBI Accession: NM_015892, NP_056976

UniProt: Q7LFX5

Pathways: Glycosaminoglycan Metabolic Process