PIGT antibody (N-Term)

Catalog No. ABIN2782864

The Rabbit Polyclonal anti-PIGT antibody is suitable to detect PIGT in samples from Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog and Horse. It has been validated for WB.

Quick Overview for PIGT antibody (N-Term) (ABIN2782864)

Target: PIGT (Phosphatidylinositol Glycan T (PIGT))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PIGT Antibody

Binding Specificity: N-Term

Purpose: PIGT Antibody - N-terminal region

Sequence: PLPSGDVAAT FQFRTRWDSE LQREGVSHYR LFPKALGQLI SKYSLRELHL

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PIGT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIGT

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47203-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PIGT

Target: PIGT (Phosphatidylinositol Glycan T (PIGT))

Alternative Name: PIGT

Background:

Background Information: PIGT is a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Phosphatidylinositol glycan anchor biosynthesis, class T

Alternative Symbols: NDAP, PNH2, CGI-06, MCAHS3

Protein Name: GPI transamidase component PIG-T

Molecular Weight: 64kDa

Gene ID: 51604

NCBI Accession: NP_057021

UniProt: Q969N2

Pathways: Inositol Metabolic Process