NAGPA antibody (N-Term)

Catalog No. ABIN2782884

Product Details anti-NAGPA Antibody

Target: NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))

Binding Specificity: N-Term

Reactivity: Human, Guinea Pig, Horse, Mouse, Cow, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAGPA antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GECLGNVVSD ERRVSSSGGL QNAQFGIRRD GTLVTGYLSE EEVLDTENPF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against NAGPA. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Human NAGPA

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NAGPA

Target: NAGPA (N-Acetylglucosamine-1-phosphodiester alpha-N-Acetylglucosaminidase (NAGPA))

Alternative Name: NAGPA (NAGPA Products)

Synonyms: NAGPA antibody, APAA antibody, UCE antibody, AI596180 antibody, N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase antibody, NAGPA antibody, NAEGRDRAFT_56913 antibody, Nagpa antibody

Background: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. This gene encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. This reaction most likely occurs in the trans-Golgi network. This enzyme functions as a homotetramer of two disulfide-linked homodimers. In addition to having an N-terminal signal peptide, the protein's C-terminus contains multiple signals for trafficking it between lysosomes, the plasma membrane, and trans-Golgi network.
Alias Symbols: APAA, UCE
Protein Interaction Partner: ZHX2, DEGS1, NAGPA, EPS15, AP2A1, FURIN,
Protein Size: 515

Molecular Weight: 53 kDa

Gene ID: 51172

NCBI Accession: NM_016256, NP_057340

UniProt: Q9UK23