KIRREL antibody (Middle Region)

Catalog No. ABIN405910

This Rabbit Polyclonal antibody specifically detects KIRREL in WB. It exhibits reactivity toward Mouse, Rat, Human, Cow, Dog, Guinea Pig, Horse and Rabbitand has been mentioned in 1 publication.

Quick Overview for KIRREL antibody (Middle Region) (ABIN405910)

Target: KIRREL (NEPH1) (Kin of IRRE Like 1 (NEPH1))

Reactivity: Mouse, Rat, Human, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIRREL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KIRREL Antibody

Binding Specificity: Middle Region

Sequence: FLEVGTLERY TVERTNSGSG VLSTLTINNV MEADFQTHYN CTAWNSFGPG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against KIRREL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KIRREL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 757 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-KIRREL antibody (ABIN405910)

Ito, Sugihara, Asaka, Toyama, Yoshihara, Furuichi, Wada, Asano: "Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice." in: PLoS ONE, Vol. 7, Issue 1, pp. e29873, (2012) (PubMed).

Target Details for KIRREL

Target: KIRREL (NEPH1) (Kin of IRRE Like 1 (NEPH1))

Alternative Name: KIRREL

Background: KIRREL (NEPH1) is a member of the nephrin-like protein family, which includes NEPH2 and NEPH3. The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2, MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-337 AY302131.1 1-337 338-3211 BC109193.1 1-2874 3212-3609 AK001707.1 1904-2301 3610-3628 BC082969.1 2273-2291
Alias Symbols: FLJ10845, MGC129542, MGC129543, NEPH1
Protein Interaction Partner: UBC, SAV1, LATS1, GRB2, KIRREL, NPHS2, NPHS1, TJP1,
Protein Size: 757

Molecular Weight: 84 kDa

Gene ID: 55243

NCBI Accession: NM_018240, NP_060710

UniProt: Q96J84

Pathways: Regulation of Actin Filament Polymerization