KIRREL antibody (Middle Region)

Catalog No. ABIN2782952

This Rabbit Polyclonal antibody specifically detects KIRREL in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit.It has been mentioned in 1 publication

Quick Overview for KIRREL antibody (Middle Region) (ABIN2782952)

Target: KIRREL (NEPH1) (Kin of IRRE Like 1 (NEPH1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIRREL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KIRREL Antibody

Binding Specificity: Middle Region

Purpose: KIRREL1 Antibody - middle region

Sequence: FLEVGTLERY TVERTNSGSG VLSTLTINNV MEADFQTHYN CTAWNSFGPG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against KIRREL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KIRREL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47408-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-KIRREL antibody (ABIN2782952)

Ito, Sugihara, Asaka, Toyama, Yoshihara, Furuichi, Wada, Asano: "Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice." in: PLoS ONE, Vol. 7, Issue 1, pp. e29873, (2012) (PubMed).

Target Details for KIRREL

Target: KIRREL (NEPH1) (Kin of IRRE Like 1 (NEPH1))

Alternative Name: KIRREL1

Background:

Background Information: NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2, MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008]

Gene Name: kirre like nephrin family adhesion molecule 1

Alternative Symbols: NEPH1, KIRREL, NPHS23

Protein Name: kin of IRRE-like protein 1

Molecular Weight: 84 kDa

Gene ID: 55243

NCBI Accession: NP_060710

UniProt: Q96J84

Pathways: Regulation of Actin Filament Polymerization