FTO antibody (N-Term)

Catalog No. ABIN2782980

This Rabbit Polyclonal antibody specifically detects FTO in WB and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Sheep, Rabbit, Pig, Horse and Saccharomyces cerevisiae.

Quick Overview for FTO antibody (N-Term) (ABIN2782980)

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Reactivity: Human, Mouse, Rat, Dog, Cow, Sheep, Rabbit, Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FTO antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-FTO Antibody

Binding Specificity: N-Term

Purpose: Fto Antibody - N-terminal region

Sequence: MKRVQTAEER EREAKKLRLL EELEDTWLPY LTPKDDEFYQ QWQLKYPKLV

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Yeast: 90%

Characteristics: This is a rabbit polyclonal antibody against Fto. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP47473-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Alternative Name: Fto

Background:

Background Information: Fto is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Fto has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Fto has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Fto has no activity towards 1-methylguanine and no detectable activity towards double-stranded DNA. Fto requires molecular oxygen, alpha-ketoglutarate and iron. Fto contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Fto contributes to the regulation of body size and body fat accumulation.

Gene Name: Fat mass and obesity associated

Alternative Symbols: AW743446, mKIAA1752

Protein Name: Alpha-ketoglutarate-dependent dioxygenase FTO

Molecular Weight: 58kDa

Gene ID: 26383

NCBI Accession: NP_036066

UniProt: Q8BGW1