14-3-3 zeta antibody (Middle Region)

Catalog No. ABIN2783207

Product Details anti-14-3-3 zeta Antibody

Target: 14-3-3 zeta (YWHAZ)

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Zebrafish (Danio rerio), Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: FDEAIAELDT LSEESYKDST LIMQLLRDNL TLWTSDTQGD EAEAGEGGEN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against YWHAZ. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human YWHAZ

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 245 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: YWHAZ (YWHAZ Products)

Background: YWHAZ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.
Alias Symbols: KCIP-1, MGC111427, MGC126532, MGC138156, YWHAD, 14-3-3-zeta
Protein Interaction Partner: FUS, UBC, HUWE1, MAPT, DTL, MAPK8, SGK1, ABL1, TSC2, DDIT4, SIK3, SIK2, SUMO2, STAU1, IRS4, MDM2, WWOX, YWHAZ, SNRPF, SNRPE, SLC9A3R1, LDHB, SFN, PFAS, PAK2, ERP44, MAPRE1, FERMT2, YWHAQ, PRMT5, YWHAH, YWHAG, VCL, UBE2V1, TGM2, EIF5, ECHS1, CAPNS1, ZFYVE1
Protein Size: 245

Molecular Weight: 28 kDa

Gene ID: 7534

NCBI Accession: NM_003406, NP_003397

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location