ALDOA antibody (N-Term)

Catalog No. ABIN2783231

Cited in 2+ publications. The Rabbit Polyclonal anti-ALDOA antibody (ABIN2783231) specifically detects ALDOA in WB. The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse, Sheep and Zebrafish (Danio rerio) samples.

Quick Overview for ALDOA antibody (N-Term) (ABIN2783231)

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDOA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALDOA Antibody

Binding Specificity: N-Term

Purpose: ALDOA Antibody - N-terminal region

Sequence: MPYQYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 93%, Sheep: 82%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against ALDOA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ALDOA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094419

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ALDOA antibody (ABIN2783231)

Braceland, McLoughlin, Tinsley, Wallace, Cockerill, McLaughlin, Eckersall: "Serum enolase: a non-destructive biomarker of white skeletal myopathy during pancreas disease (PD) in Atlantic salmon Salmo salar L." in: Journal of fish diseases, Vol. 38, Issue 9, pp. 821-31, (2015) (PubMed).

Mao, Yang, Xu, Sang, Lu, Yang, Zhang, Zhong, Huang, Zhang: "[Significance of Golgi glycoprotein 73, a new tumor marker in diagnosis of hepatocellular carcinoma: a primary study]." in: Zhonghua yi xue za zhi, Vol. 88, Issue 14, pp. 948-51, (2008) (PubMed).

Target Details for ALDOA

Target: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Alternative Name: ALDOA

Background:

Background Information: ALDOA is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Gene Name: Aldolase A, fructose-bisphosphate

Alternative Symbols: ALDA, GSD12, HEL-S-87p

Protein Name: Fructose-bisphosphate aldolase A

Molecular Weight: 39 kDa

Gene ID: 226

NCBI Accession: NP_000025

UniProt: P04075

Pathways: Ribonucleoside Biosynthetic Process