EIF3E antibody (Middle Region)

Catalog No. ABIN2783260

The Rabbit Polyclonal anti-EIF3E antibody has been validated for WB and IHC. It is suitable to detect EIF3E in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse and Rabbit.

Quick Overview for EIF3E antibody (Middle Region) (ABIN2783260)

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EIF3E Antibody

Binding Specificity: Middle Region

Sequence: LNMTPEEAER WIVNLIRNAR LDAKIDSKLG HVVMGNNAVS PYQQVIEKTK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF3E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF3E

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 445 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for EIF3E

Target: EIF3E (Eukaryotic Translation Initiation Factor 3 Subunit E (EIF3E))

Alternative Name: EIF3E

Background: EIF3E belongs to the eIF-3 subunit E family.It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. EIF3E is required for nonsense-mediated mRNA decay (NMD), It may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. The protein may interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
Alias Symbols: EIF3-P48, EIF3S6, INT6, eIF3-p46
Protein Interaction Partner: UBC, C4orf19, RUNDC3A, HUWE1, TRIM63, TRIM55, STAU1, SUMO1, NEDD8, GPRASP2, LARP1, GNB2L1, RPS28, RPS25, RPS23, RPS20, RPS19, RPS18, RPS12, RPS10, RPS9, RPS8, RPS3A, RPS3, RPS2, EIF3CL, DYNC1LI1, FBXO6, EIF3D, IGSF8, NPM1, ICAM1, CD81, EIF3B, VCAM1, POLR2
Protein Size: 445

Molecular Weight: 52 kDa

Gene ID: 3646

NCBI Accession: NM_001568, NP_001559

UniProt: P60228

Pathways: Ribonucleoprotein Complex Subunit Organization, Hepatitis C