APEH antibody (N-Term)

Catalog No. ABIN2783264

This Rabbit Polyclonal antibody specifically detects APEH in WB, IHC and IP. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig, Cow, Horse, Rabbit, Zebrafish (Danio rerio) and Dog.

Quick Overview for APEH antibody (N-Term) (ABIN2783264)

Target: APEH (N-Acylaminoacyl-Peptide Hydrolase (APEH))

Reactivity: Human, Mouse, Rat, Guinea Pig, Cow, Horse, Rabbit, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-APEH Antibody

Binding Specificity: N-Term

Purpose: APEH Antibody - N-terminal region

Sequence: VYEDDCFGCL SWSHSETHLL YVAEKKRPKA ESFFQTKALD VSASDDEIAR

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 83%

Characteristics: This is a rabbit polyclonal antibody against APEH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human APEH

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48181-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for APEH

Target: APEH (N-Acylaminoacyl-Peptide Hydrolase (APEH))

Alternative Name: APEH

Background:

Background Information: APEH is the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: N-acylaminoacyl-peptide hydrolase

Alternative Symbols: APH, OPH, AARE, ACPH, D3S48E, D3F15S2, DNF15S2

Protein Name: Acylamino-acid-releasing enzyme

Molecular Weight: 81kDa

Gene ID: 327

NCBI Accession: NP_001631

UniProt: P13798