ATP5B antibody (C-Term)

Catalog No. ABIN2783269

This Rabbit Polyclonal antibody specifically detects ATP5B in WB, IHC and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae and Guinea Pig and has been mentioned in 3+ publications.

Quick Overview for ATP5B antibody (C-Term) (ABIN2783269)

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-ATP5B Antibody

Binding Specificity: C-Term

Sequence: MGKLVPLKET IKGFQQILAG EYDHLPEQAF YMVGPIEEAV AKADKLAEEH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 77%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against ATP5B. It was validated on Western Blot using a cell lysate as a positive control. Catalog ABIN973616 can be used a blocking peptide for this product.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ATP5B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 529 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATP5B antibody (ABIN2783269)

Usui, Kajita, Kajita, Mori, Hanamoto, Ikeda, Okada, Taguchi, Kitada, Morita, Sasaki, Kitamura, Sato, Kojima, Ishizuka: "Elevated mitochondrial biogenesis in skeletal muscle is associated with testosterone-induced body weight loss in male mice." in: FEBS letters, Vol. 588, Issue 10, pp. 1935-41, (2014) (PubMed).

Rubel, Schisler, Hamlett, DeKroon, Gautel, Alzate, Patterson: "Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates." in: Cell biochemistry and biophysics, Vol. 67, Issue 1, pp. 127-38, (2013) (PubMed).

Teodoro, Duarte, Gomes, Varela, Peixoto, Rolo, Palmeira: "Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation." in: Mitochondrion, Vol. 13, Issue 6, pp. 637-46, (2013) (PubMed).

Target Details for ATP5B

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Alternative Name: ATP5B

Background: ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5B is the beta subunit of the catalytic core.This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ATPMB, ATPSB, MGC5231
Protein Interaction Partner: FUS, FDX1, ATP5A1, HUWE1, ATPAF2, TRIM63, TRIM55, SPRTN, STAU1, GRSF1, UBC, MDM2, ASB5, ASB11, SUZ12, RNF2, EZH2, BMI1, GBP1, ADRB2, TERT, vpu, UBL4A, WHSC1, VCAM1, PPP1CC, ITGA4, FN1, ESR1, ATF2, BTK, YWHAE, SUMO2, CA9, FMNL1, ATP5O, ATP6V1B2, ATP5C1, AT
Protein Size: 529

Molecular Weight: 52 kDa

Gene ID: 506

NCBI Accession: NM_001686, NP_001677

UniProt: P06576

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process