BAG3 antibody (Middle Region)

Catalog No. ABIN2783302

The Rabbit Polyclonal anti-BAG3 antibody is suitable to detect BAG3 in samples from Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse and Dog. It has been validated for WB.

Quick Overview for BAG3 antibody (Middle Region) (ABIN2783302)

Target: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAG3 Antibody

Binding Specificity: Middle Region

Purpose: BAG3 Antibody - middle region

Sequence: NVTRPAAQPS FHQAQKTHYP AQQGEYQTHQ PVYHKIQGDD WEPRPLRAAS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against BAG3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human BAG3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48251-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for BAG3

Target: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Alternative Name: BAG3

Background:

Background Information: BAG3 inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. BAG3 has anti-apoptotic activity.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: BCL2-associated athanogene 3

Alternative Symbols: BIS, MFM6, BAG-3, CAIR-1

Protein Name: BAG family molecular chaperone regulator 3

Molecular Weight: 61kDa

Gene ID: 9531

NCBI Accession: NP_004272

UniProt: O95817