HPRT1 antibody (Middle Region)

Catalog No. ABIN2783383

The Rabbit Polyclonal anti-HPRT1 antibody (ABIN2783383) specifically detects HPRT1 in WB. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Guinea Pig, Horse and Rabbit samples.

Quick Overview for HPRT1 antibody (Middle Region) (ABIN2783383)

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPRT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HPRT1 Antibody

Binding Specificity: Middle Region

Purpose: HPRT1 Antibody - middle region

Sequence: STGDIKVIGG DDLSTLTGKN VLIVEDIIDT GKTMQTLLSL VRQYNPKMVK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against HPRT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HPRT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8102911

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HPRT1

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HPRT1

Background:

Background Information: HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate.HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Hypoxanthine phosphoribosyltransferase 1

Alternative Symbols: HPRT, HGPRT

Protein Name: Hypoxanthine-guanine phosphoribosyltransferase

Molecular Weight: 25 kDa

Gene ID: 3251

NCBI Accession: NP_000185

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process