Dnmt2 antibody (N-Term)

Catalog No. ABIN2783440

The Rabbit Polyclonal anti-Dnmt2 antibody (ABIN2783440) specifically detects Dnmt2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig and Horse samples.

Quick Overview for Dnmt2 antibody (N-Term) (ABIN2783440)

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dnmt2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Dnmt2 Antibody

Binding Specificity: N-Term

Purpose: TRDMT1 Antibody - N-terminal region

Sequence: MILMSPPCQP FTRIGRQGDM TDSRTNSFLH ILDILPRLQK LPKYILLENV

Predicted Reactivity: Cow: 100%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TRDMT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRDMT1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48627-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Dnmt2

Target: Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))

Alternative Name: TRDMT1

Background:

Background Information: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. TRDMT1 is a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome.CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a protein with similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Name: TRNA aspartic acid methyltransferase 1

Alternative Symbols: DMNT2, DNMT2, PUMET, RNMT1, MHSAIIP

Protein Name: tRNA (cytosine(38)-C(5))-methyltransferase

Molecular Weight: 45 kDa

Gene ID: 1787

NCBI Accession: NP_004403

UniProt: O14717