NID2 antibody (Middle Region)

Catalog No. ABIN502977

The Rabbit Polyclonal anti-NID2 antibody (ABIN502977) specifically detects NID2 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog and Horse samples.

Quick Overview for NID2 antibody (Middle Region) (ABIN502977)

Target: NID2 (Nidogen 2 (NID2))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NID2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NID2 Antibody

Binding Specificity: Middle Region

Purpose: NID2 Antibody - middle region

Sequence: DDLGHFIPLQ CHGKSDFCWC VDKDGREVQG TRSQPGTTPA CIPTVAPPMV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 85%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NID2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NID2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48729-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NID2

Target: NID2 (Nidogen 2 (NID2))

Alternative Name: NID2

Background:

Background Information: Basement membranes, which are composed of type IV collagens, laminins, perlecan, and nidogen, are thin pericellular protein matrices that control a large number of cellular activities, including adhesion, migration, differentiation, gene expression, and apoptosis.Basement membranes, which are composed of type IV collagens (see MIM 120130), laminins (see LAMC1, MIM 150290), perlecan (HSPG2, MIM 142461), and nidogen (see NID1, MIM 131390), are thin pericellular protein matrices that control a large number of cellular activities, including adhesion, migration, differentiation, gene expression, and apoptosis.[supplied by OMIM].

Gene Name: Nidogen 2 (osteonidogen)

Alternative Symbols: NID-2

Protein Name: Nidogen-2

Molecular Weight: 148kDa

Gene ID: 22795

NCBI Accession: NP_031387

UniProt: Q14112