POLR3B antibody (C-Term)

Catalog No. ABIN2783516

The Rabbit Polyclonal anti-POLR3B antibody has been validated for WB. It is suitable to detect POLR3B in samples from Human, Mouse, Rat, Dog, Cow, Rabbit, Zebrafish (Danio rerio), Horse, Guinea Pig, Saccharomyces cerevisiae and Goat. There is 1 publication available.

Quick Overview for POLR3B antibody (C-Term) (ABIN2783516)

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Zebrafish (Danio rerio), Horse, Guinea Pig, Saccharomyces cerevisiae, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR3B Antibody

Binding Specificity: C-Term

Sequence: IEKVMISSNA EDAFLIKMLL RQTRRPEIGD KFSSRHGQKG VCGLIVPQED

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against POLR3B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human POLR3B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1133 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-POLR3B antibody (ABIN2783516)

Yee, Gong, Huang, Lorent, Dolan, Maraia, Pack: "Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development." in: PLoS biology, Vol. 5, Issue 11, pp. e312, (2007) (PubMed).

Target Details for POLR3B

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Alternative Name: POLR3B

Background: POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Alias Symbols: FLJ10388, RPC2, C128, HLD8
Protein Interaction Partner: SUMO2, UBC, POLR3H, TSR1, POLR1D, CRCP, POLR3A, POLR1C, POLR2H, POLR3D, RPAP2, RPAP1, GPN1, RAE1,
Protein Size: 1133

Molecular Weight: 128 kDa

Gene ID: 55703

NCBI Accession: NM_018082, NP_060552

UniProt: Q9NW08