POLR1B antibody (Middle Region)
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- Target See all POLR1B Antibodies
- POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))
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Binding Specificity
- Middle Region
- Reactivity
- Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Pig, Cow, Dog, Guinea Pig, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLR1B antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- SDKFQVRTTG ARDRVTNQPI GGRNVQGGIR FGEMERDALL AHGTSFLLHD
- Predicted Reactivity
- Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 86%
- Characteristics
- This is a rabbit polyclonal antibody against POLR1B. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human POLR1B
- Top Product
- Discover our top product POLR1B Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 1135 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))
- Alternative Name
- POLR1B (POLR1B Products)
- Synonyms
- RPOL-1l antibody, MGC68946 antibody, 128kDa antibody, D630020H17Rik antibody, RPA116 antibody, RPA135 antibody, RPA2 antibody, Rpo1-2 antibody, RNA polymerase I subunit B antibody, polymerase (RNA) I polypeptide B L homeolog antibody, polymerase (RNA) I polypeptide B, 128kDa antibody, polymerase (RNA) I polypeptide B antibody, DNA-directed RNA polymerase I subunit RPA2 antibody, POLR1B antibody, polr1b.L antibody, polr1b antibody, LOC100563266 antibody, LOC100634236 antibody, Polr1b antibody
- Background
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Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).
Alias Symbols: FLJ10816, FLJ21921, MGC131780, RPA135, Rpo1-2, RPA2
Protein Interaction Partner: UBC, RPA3, RPA2, RPA1, RRN3, HDAC6, GIT2, MED20, NMI, LDB1, ZNF207, RPL37, CCND1, AKT2, ACTA1, ERBB2, ACTB, TSR1, POLR1D, POLR1A, CD3EAP, NOP56, POLR1C, DHX8, CAND1, ARRB2, LZTR1, TAF1D, USP11, POLR1E, EIF3L, NFKBIB, POLR1B, ESR1, RIBIN,
Protein Size: 1135 - Molecular Weight
- 128 kDa
- Gene ID
- 84172
- NCBI Accession
- NM_019014, NP_061887
- UniProt
- Q9H9Y6
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