CHST14 antibody (Middle Region)

Catalog No. ABIN2783597

There is 1 publication for this product available. The Rabbit Polyclonal anti-CHST14 antibody is suitable to detect CHST14 in samples from Human, Mouse, Rat, Cow, Dog, Pig and Guinea Pig. It has been validated for WB.

Quick Overview for CHST14 antibody (Middle Region) (ABIN2783597)

Target: CHST14 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 14 (CHST14))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHST14 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CHST14 Antibody

Binding Specificity: Middle Region

Purpose: CHST14 Antibody - middle region

Sequence: REYQQRYGAE IVRRYRAGAG PSPAGDDVTF PEFLRYLVDE DPERMNEHWM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CHST14. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CHST14

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48994-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHST14 antibody (ABIN2783597)

Janecke, Li, Boehm, Krabichler, Rohrbach, Müller, Fuchs, Golas, Katagiri, Ziegler, Gahl, Wilnai, Zoppi, Geller, Giunta, Slavotinek, Steinmann: "The phenotype of the musculocontractural type of Ehlers-Danlos syndrome due to CHST14 mutations." in: American journal of medical genetics. Part A, Vol. 170A, Issue 1, pp. 103-15, (2016) (PubMed).

Target Details for CHST14

Target: CHST14 (Carbohydrate (N-Acetylgalactosamine 4-0) Sulfotransferase 14 (CHST14))

Alternative Name: CHST14

Background:

Background Information: CHST14 belongs to the sulfotransferase 2 family. CHST14 catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. It transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. It transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. CHST14 has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA.

Gene Name: Carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14

Alternative Symbols: ATCS, D4ST1, EDSMC1, HNK1ST

Protein Name: Carbohydrate sulfotransferase 14

Molecular Weight: 35kDa

Gene ID: 113189

UniProt: Q8NCH0

Pathways: Glycosaminoglycan Metabolic Process